Cloning, Expression and Purification of Proteins

Cleanergis has capabilities for characterizing some of the potential microorganisms for Enzyme studies. The proteins have been expressed for being potential as high order enzymes. These studies are aimed at cloning, expression, and purification of several such proteins. Recombinant plasmids are introduced into Escherichia coli and the recombinant proteins are purified from the cytosolic fractions of the E. coli sonicates. The purity, molecular mass, and the conformation of the proteins can be determined by high performance liquid chromatography (HPLC), matrix assisted laser desorption-ionization-time-of-flight (MALDI-TOF), and circular dichroism (CD) studies.